牦牛和雄性不育犏牛睾丸FABP5和FABP9基因mRNA水平及能量代谢相关酶活力的比较

doi:10.11843/j.issn.0366-6964.2015.04.008

Abstract

Abstract:

The epidermal type fatty acid binding protein (FABP5) and testicular fatty acid binding protein (FABP9) genes of yak were sequenced，and their expressions as well as activities of some enzymes related to energy metabolism in the testes were compared between yak and sterile F1 males (male cattle × female yak)，aiming to explore the association between the two genes and energy metabolism with the sterility of F1 males.Total RNA was extracted from yak testes and the cDNA sequences of FABP5 and FABP9 genes were obtained using conventional PCR methods.The coding region of yak FABP5 and FABP9 genes were 408 and 399 bp，respectively，and showed 1 and 6 nucleotide differences compared with those of cattle，respectively，the latter caused 5 amino acid substitutions in the deduced amino acid sequences.Real-time quantitative PCR analysis showed that the expression of FABP5 gene in the sterile F1 testes was significantly greater than yak，but not for FABP9 gene.Isocitrate dehydrogenase activity in sterile F1 testes was significantly higher than yak (P<0.01)，while β-hydroxyacyl CoA dehydrogenase and lactate dehydrogenase activities were similar in the testes of yak and sterile F1 males.The up-regulated expression of FABP5 gene and the increased activity of isocitrate dehydrogenase involved in citric acid cycle in the sterile F1 testes may indicate that the level of fatty acid oxidation for energy is higher in the testes of sterile F1 males than adult yak.

CLC Number:

S823.8

FU Wei，HUANG Lin，LIU Wen-jing，REN Liang，LI Cai-xia，JIN Su-yu，LIN Ya-qiu，ZHENG Yu-cai. Comparison of the mRNA Levels of FABP5 and FABP9 Genes and Activities of Some Enzymes Related to Energy Metabolism in the Testes of Yak and Sterile F1 Male Hybrids between Male Cattle and Female Yak[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(4): 561-567.

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References

［1］CHMURZY?SKA A.The multigene family of fatty acid-binding proteins (FABPs)：function，structure and polymorphism［J］.J Appl Genet，2006，47(1)：39-48.
［2］ZIMMERMAN A W，VEERKAMP J H.New insights into the structure and function of fatty acid-binding proteins［J］.Cell Mol Life Sci，2002，59(7)：1096-1116.
［3］SANSON B，WANG T，SUN J，et al.Crystallographic study of FABP5 as an intracellular endocannabinoid transporter［J］.Acta Crystallogr D Biol Crystallogr，2014，70(Pt 2):290-298.
［4］ZHANG Y，SUN Y，RAO E，et al.Fatty acid binding protein E-FABP restricts tumor growth by promoting IFNβ responses in tumor-associated macrophages ［J］.Cancer Res，2014，74(11):2986-2998.
［5］KORLEY R，POURESMAEILI F，OKO R.Analysis of the protein composition of the mouse sperm perinuclear theca and characterization of its major protein constituent［J］.Biol Reprod，1997，57(6)：1426-1432.
［6］KIDO T，ARATA S，SUZUKI R，et al.The testicular fatty acid binding protein PERF15 regulates the fate of germ cells in PERF15 transgenic mice［J］.Dev Growth Differ，2005，47(1)：15-24.
［7］OKO R，MORALES C R.A novel testicular protein，with sequence similarities to a family of lipid binding proteins，is a major component of the rat sperm perinuclear theca［J］.Dev Biol，1994，166(1)：235-245.
［8］张旭静.牦牛和普通牛种间杂种公牛睾丸的组织学观测与研究［J］.畜牧兽医学报，2001，32(4)：314-318.
ZHANG X J.An observation and study of testicular histology in hybrid bull copulated by yak and cattle［J］.Acta Veterinaria et Zootechnica Sinica，2001，32(4)：314-318.(in Chinese)
［9］屈旭光，李齐发，刘振山，等.牦牛、犏牛睾丸组织中SYCP3基因mRNA表达水平研究［J］.畜牧兽医学报，2008，39(8)：1132-1136.
QU X G，LI Q F，LIU Z S，et al.The study on the expression level of SYCP3 mRNA in yak and cattle-yak testis［J］.Acta Veterinaria et Zootechnica Sinica，2008，39(8)：1132-1136.(in Chinese)
［10］GU Y，LI Q，PAN Z，et al.Molecular cloning，gene expression and methylation status analysis of PIWIL1 in cattle-yaks and the parental generation［J］.Anim Reprod Sci，2013，140(3-4)：131-137.
［11］金帅，郭宪，包鹏甲，等.牦牛和犏牛Dmrt7基因序列分析及其在睾丸组织中的表达水平［J］.中国农业科学，2013，46(5)：1036-1043.
JIN S，GUO X，BAO P J，et al.Sequence analysis and study on the expression level of Dmrt7 gene in yak and cattle-yak testis［J］.Scientia Agricultura Sinica，2013，46(5)：1036-1043.(in Chinese)
［12］LUO H，ZHOU Y，LI Y，et al.Splice variants and promoter methylation status of the bovine vasa homology (Bvh) gene may be involved in bull spermatogenesis［J］.BMC Genet，2013，14(1):58.doi:10.1186/1471-2156-14-58.
［13］付伟，李彩霞，刘文静，等.利用双向电泳-质谱技术鉴定牦牛和犏牛摹丸中的差异表达蛋白质［J］.西南民族大学学报:自然科学版，2014，40(1)：11-15.
FU W，LI C X，LIU W J，et al.Identification of the differentially expressed testis proteins between yaks and cattle-yaks by two-dimensional electrophoresis combined with mass spectrometry［J］.Journal of Southwest University for Nationalities，Natural Science Edition，2014，40(1)：11-15.(in Chinese)
［14］邱翔，林亚秋，王国生，等.牦牛睾丸组织能量代谢相关酶活力的分析［J］.四川动物，2010，29(5)：590-592.
QU X，LIN Y Q，WANG G S，et al.Analysis of energy metabolism-related enzyme activities in yak testis tissue［J］.Sichuan Journal of Zoology，2010，29(5)：590-592.(in Chinese)
［15］JURIE C，ORTIGUES-MARTY I，PICARD B，et a1.The separate effects of the nature of diet and grazing mobility on metabolic potential of muscles from Charolais steers［J］. Livest Sci，2006，104(1-2)：182-192.
［16］MARCHAT L，LOISEAU P M，PETEK F.Purification and characterization of lactate dehydrogenase isoenzyines 1 and 2 from Molinema dessetae (Nematoda：Filarioidea)［J］.Parasitol Res，1996，82(8)：672-680.
［17］BAI W L，YIN R H，ZHENG Y C，et al.Cloning and molecular characterization of a yak α-lactalbumin cDNA from mammary tissue［J］.Livest Sci，2010，129(1-3)：122-128.
［18］DING Y，XU Y O，LIN Y Q，et al.Cloning and expression profiles of yak lipoprotein lipase gene［J］. J Applied Anim Res，2012，40(4)：311-315.
［19］KINGMA P B，BOK D，ONG D E.Bovine epidermal fatty acid-binding protein：determination of ligand specificity and cellular localization in retina and testis［J］.Biochemistry，1998，37(10)：3250-3257.
［20］MAEDA K，UYSAL K T，MAKOWSKI L，et al.Role of the fatty acid binding protein mall in obesity and insulin resistance［J］.Diabetes，2003，52(2)：300-307.
［21］FURUHASHI M，HOTAMISLIGIL G S.Fatty acid-binding proteins：role in metabolic diseases and potential as drug targets［J］.Nat Rev Drug Discov，2008，7(6)：489-503.
［22］AMORY J K，ARNOLD S，LARDONE M C，et al.Levels of the retinoic acid synthesizing enzyme aldehyde dehydrogenase-1A2 are lower in testicular tissue from men with infertility［J］.Fertil Steril，2014，101(4):960-966.
［23］FUKASAWA M，ATSUZAWA K，MIZUTANI K，et al．Immunohistochemical localization of mitoehondrial fatty acid β-oxidation enzymes in rat testis［J］.J Histochem Cytochem，2010，58(2)：195-206.
［24］BAJPAI M，GUPTA G，JAIN S K.Lipid metabolising enzymes in isolated rat testicular germ cells and changes associated with meiosis［J］.Andrologia，1998，30(6)：311-315.
［25］XIONG W，WANG H，WU H，et al.Apoptotic spermatogenic cells can be energy sources for Sertoli cells［J］. Reproduction， 2009，137(3)：469-479.
［26］AMARAL A，CASTILLO J，ESTANYOL J M，et al.Human sperm tail proteome suggests new endogenous metabolic pathways［J］.Mol Cell Proteomics，2013，12(2)：330-342.
［27］BAJPAI M，GUPTA G，SETTY B S.Changes in carbohydrate metabolism of testicular germ cells during meiosis in the rat［J］.Eur J Endocrinol，1998，38(3)：322-327.
［28］LOU Y N，LIU W J，WANG C L，et al.Histological evaluation and Prdm9 expression level in the testis of sterile male cattle-yaks［J］.Livest Sci，2014，160：208-213.
［29］JAMSHIDI J，POURESMAEILI F，DARVISH H，et al.FABP9 mutations are not detected in cases of infertility due to sperm morphological defects in Iranian men［J］. Int J Fertil Steril，2014，7(4)：275-280.
［30］SELVARAJ V，ASANO A，PAGE J L，et al.Mice lacking FABP9/PERF15 develop sperm head abnormalities but are fertile［J］.Dev Biol，2010，348(2)：177-189.

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Comparison of the mRNA Levels of FABP5 and FABP9 Genes and Activities of Some Enzymes Related to Energy Metabolism in the Testes of Yak and Sterile F1 Male Hybrids between Male Cattle and Female Yak

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